A magnitude 3.0 tremor on Monday rattled quake-hit central Italy with an epicentre in the Umbrian province of Perugia, Italy's national institute of geophysics and vulcanology reported.
The quake struck at 2.32 am and the towns closest to its epicentre were Campello sul Clitunno, Spoleto and Castel Ritaldi, the institute said.
Three further tremors of between magnitude 2.2 and 2.6 were registered in between 4.26 am and 5.01 am in the border region between Lazio, Umbria and Marche, according to the institute.
A major 6.0-magnitude temblor that struck the region on 24 August last year, killing 300 people and flattening medieval hill towns.
Since then, several powerful quakes have rocked the region, leaving thousands of people homeless and further damaging buildings including medieval churches and monuments.
The earthquakes have cost the state more than 23 billion euros, Italy's civil protection agency said in February.
Thousands of tremors have continued to bedevil mountainous areas of central Italy over the past seven months.